Storage temperature: The buns were stored at room temperature after production. This allows a wide range of microorganisms such as psychrophiles and mesophiles to grow.
Gaseous environment: The packaging material used to pack the buns is LDPE plastics. Although this type of packaging is a good water barrier, however, it is a poor gas barrier. Therefore, undesirable gases such as oxygen can enter the packaging. This makes it possible for microbes such as facultative aerobes to grow.
Relative Humidity (RH): The RH of the buns during the proofing is 90%. This helps to ferment the yeast better but at the same time, it encourages the growth of molds.